| pepnet

## Function

Displays proteins as a helical net

## Description

This is a method of displaying the residues of a protein in a simple 3,4,3,4 repeating pattern that emulates at a simple level the arrangement of residues around an alpha helix.

It is therefore easy to see patterns of amphipathicity that you may wish to investigate in more detail by using displays such as pepwheel.

You can specify which residues to mark up in squares, diamonds and octagons.

## Usage

Here is a sample session with pepnet

    % pepnet -sask
    Displays proteins as a helical net
    Input sequence: tsw:gcn4_yeast
         Begin at position [start]: 253
           End at position [end]: 274
    Graph type [x11]: cps

    Created pepnet.ps

Go to the input files for this example
Go to the output files for this example

## Command line arguments

       Mandatory qualifiers (* if not always prompted):
      [-sequence]          sequence   Sequence USA
    *  -graph              graph      Graph type

       Optional qualifiers (* if not always prompted):
    *  -squares            string     By default the aliphatic residues ILVM are
                                      marked with squares.
    *  -diamonds           string     By default the residues DENQST are marked
                                      with diamonds.
    *  -octags             string     By default the positively charged residues
                                      HKR are marked with octagons.

       Advanced qualifiers:
       -amphipathic        boolean    If this is true then the residues ACFGILMVWY
                                      are marked as squares and all other
                                      residues are unmarked. This overrides any
                                      other markup that you may have specified
                                      using the qualifiers '-squares', '-diamonds'
                                      and '-octags'.
       -data               boolean    Output the data to a file instead of
                                      plotting it

       Associated qualifiers:
      "-sequence" related qualifiers
       -sbegin1             integer    First base used
       -send1               integer    Last base used, def=seq length
       -sreverse1           boolean    Reverse (if DNA)
       -sask1               boolean    Ask for begin/end/reverse
       -snucleotide1        boolean    Sequence is nucleotide
       -sprotein1           boolean    Sequence is protein
       -slower1             boolean    Make lower case
       -supper1             boolean    Make upper case
       -sformat1            string     Input sequence format
       -sopenfile1          string     Input filename
       -sdbname1            string     Database name
       -sid1                string     Entryname
       -ufo1                string     UFO features
       -fformat1            string     Features format
       -fopenfile1          string     Features file name
      "-graph" related qualifiers
       -gprompt             boolean    Graph prompting
       -gtitle              string     Graph title
       -gsubtitle           string     Graph subtitle
       -gxtitle             string     Graph x axis title
       -gytitle             string     Graph y axis title
       -goutfile            string     Output file for non interactive displays
       -gdirectory          string     Output directory

       General qualifiers:
       -auto                boolean    Turn off prompts
       -stdout              boolean    Write standard output
       -filter              boolean    Read standard input, write standard output
       -options             boolean    Prompt for required and optional values
       -debug               boolean    Write debug output to program.dbg
       -acdlog              boolean    Write ACD processing log to program.acdlog
       -acdpretty           boolean    Rewrite ACD file as program.acdpretty
       -acdtable            boolean    Write HTML table of options
       -verbose             boolean    Report some/full command line options
       -help                boolean    Report command line options. More
                                      information on associated and general
                                      qualifiers can be found with -help -verbose
       -warning             boolean    Report warnings
       -error               boolean    Report errors
       -fatal               boolean    Report fatal errors
       -die                 boolean    Report deaths

Mandatory qualifiers | Allowed values | Default
--- | --- | ---
[-sequence] (Parameter 1) | Sequence USA | Readable sequence | Required
-graph | Graph type | EMBOSS has a list of known devices, including postscript, ps, hpgl, hp7470, hp7580, meta, colourps, cps, xwindows, x11, tektronics, tekt, tek4107t, tek, none, null, text, data, xterm, png | EMBOSS_GRAPHICS value, or x11
Optional qualifiers | Allowed values | Default
-squares | By default the aliphatic residues ILVM are marked with squares. | Any string is accepted | ILVM
-diamonds | By default the residues DENQST are marked with diamonds. | Any string is accepted | DENQST
-octags | By default the positively charged residues HKR are marked with octagons. | Any string is accepted | HKR
Advanced qualifiers | Allowed values | Default
-amphipathic | If this is true then the residues ACFGILMVWY are marked as squares and all other residues are unmarked. This overrides any other markup that you may have specified using the qualifiers '-squares', '-diamonds' and '-octags'. | Boolean value Yes/No | No
-data | Output the data to a file instead of plotting it | Boolean value Yes/No | No

## Input file format

pepnet reads any protein sequence USA.

### Input files for usage example

'tsw:gcn4_yeast' is a sequence entry in the example protein database 'tsw'

### Database entry: tsw:gcn4_yeast

    ID   GCN4_YEAST     STANDARD;      PRT;   281 AA.
    AC   P03069; P03068;
    DT   21-JUL-1986 (Rel. 01, Created)
    DT   21-JUL-1986 (Rel. 01, Last sequence update)
    DT   15-JUL-1999 (Rel. 38, Last annotation update)
    DE   GENERAL CONTROL PROTEIN GCN4 (AMINO ACID BIOSYNTHESIS REGULATORY
    DE   PROTEIN).
    GN   GCN4 OR ARG9 OR AAS3 OR YEL009C.
    OS   Saccharomyces cerevisiae (Baker's yeast).
    OC   Eukaryota; Fungi; Ascomycota; Hemiascomycetes; Saccharomycetales;
    OC   Saccharomycetaceae; Saccharomyces.
    RN   [1]
    RP   SEQUENCE FROM N.A.
    RX   MEDLINE; 85038531.
    RA   HINNEBUSCH A.G.;
    RT   "Evidence for translational regulation of the activator of general
    RT   amino acid control in yeast.";
    RL   Proc. Natl. Acad. Sci. U.S.A. 81:6442-6446(1984).
    RN   [2]
    RP   SEQUENCE FROM N.A.
    RX   MEDLINE; 84298088.
    RA   THIREOS G., PENN M.D., GREER H.;
    RT   "5' untranslated sequences are required for the translational control
    RT   of a yeast regulatory gene.";
    RL   Proc. Natl. Acad. Sci. U.S.A. 81:5096-5100(1984).
    RN   [3]
    RP   SEQUENCE FROM N.A.
    RC   STRAIN=S288C / AB972;
    RA   DIETRICH F.S., MULLIGAN J.T., HENNESSEY K.M., ALLEN E., ARAUJO R.,
    RA   AVILES E., BERNO A., BRENNAN T., CARPENTER J., CHEN E., CHERRY J.M.,
    RA   CHUNG E., DUNCAN M., GUZMAN E., HARTZELL G., HUNICKE-SMITH S.,
    RA   HYMAN R., KAYSER A., KOMP C., LASHKARI D., LEW H., LIN D.,
    RA   MOSEDALE D., NAKAHARA K., NAMATH A., NORGREN R., OEFNER P., OH C.,
    RA   PETEL F.X., ROBERTS D., SEHL P., SCHRAMM S., SHOGREN T., SMITH V.,
    RA   TAYLOR P., WEI Y., YELTON M., BOTSTEIN D., DAVIS R.W.;
    RL   Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases.
    RN   [4]
    RP   DOMAINS.
    RX   MEDLINE; 87002456.
    RA   HOPE I.A., STRUHL K.;
    RT   "Functional dissection of a eukaryotic transcriptional activator
    RT   protein, GCN4 of yeast.";
    RL   Cell 46:885-894(1986).
    RN   [5]
    RP   X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 250-281.
    RX   MEDLINE; 92054531.
    RA   O'SHEA E.K., KLEMM J.D., KIM P.S., ALBER T.;
    RT   "X-ray structure of the GCN4 leucine zipper, a two-stranded, parallel
    RT   coiled coil.";
    RL   Science 254:539-544(1991).

      [Part of this file has been deleted for brevity]

    CC   -!- SUBCELLULAR LOCATION: NUCLEAR.
    CC   -!- SIMILARITY: TO OTHER BZIP PROTEINS.
    CC   --------------------------------------------------------------------------
    CC   This SWISS-PROT entry is copyright. It is produced through a collaboration
    CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
    CC   the European Bioinformatics Institute.  There are no  restrictions on  its
    CC   use  by  non-profit  institutions as long  as its content  is  in  no  way
    CC   modified and this statement is not removed.  Usage  by  and for commercial
    CC   entities requires a license agreement (See http://www.isb-sib.ch/announce/
    CC   or send an email to license@isb-sib.ch).
    CC   --------------------------------------------------------------------------
    DR   EMBL; K02205; AAA34640.1; -.
    DR   EMBL; K02649; AAA65521.1; -.
    DR   EMBL; U18530; AAB64486.1; -.
    DR   PIR; A03604; RGBYA1.
    DR   PIR; A03605; RGBYA2.
    DR   PDB; 1ZTA; 15-APR-93.
    DR   PDB; 2ZTA; 15-OCT-92.
    DR   PDB; 1YSA; 31-OCT-93.
    DR   PDB; 1DGC; 22-JUN-94.
    DR   PDB; 2DGC; 08-MAR-96.
    DR   PDB; 1GCL; 03-JUN-95.
    DR   PDB; 1GCM; 29-JAN-96.
    DR   PDB; 1SWI; 08-NOV-96.
    DR   PDB; 1ZII; 07-JUL-97.
    DR   PDB; 1ZIJ; 07-JUL-97.
    DR   PDB; 1ZIK; 07-JUL-97.
    DR   PDB; 1ZIL; 07-JUL-97.
    DR   PDB; 1ZIM; 07-JUL-97.
    DR   PDB; 1PIQ; 30-SEP-98.
    DR   PDB; 1CE9; 25-MAR-99.
    DR   TRANSFAC; T00321; -.
    DR   SGD; L0000683; GCN4.
    DR   PFAM; PF00170; bZIP; 1.
    DR   PROSITE; PS00036; BZIP_BASIC; 1.
    KW   Amino-acid biosynthesis; Transcription regulation; Activator;
    KW   DNA-binding; Nuclear protein; 3D-structure.
    FT   DOMAIN      106    125       REQUIRED FOR TRANSCRIPTIONAL ACTIVATION.
    FT   DNA_BIND    231    249       BASIC MOTIF.
    FT   DOMAIN      253    274       LEUCINE-ZIPPER.
    FT   CONFLICT    239    281       ARRSRARKLQRMKQLEDKVEELLSKNYHLENEVARLKKLVG
    FT                                ER -> PGVLVRESCKE (IN REF. 2).
    FT   HELIX       227    276
    FT   TURN        277    277
    SQ   SEQUENCE   281 AA;  31310 MW;  A2155238 CRC32;
         MSEYQPSLFA LNPMGFSPLD GSKSTNENVS ASTSTAKPMV GQLIFDKFIK TEEDPIIKQD
         TPSNLDFDFA LPQTATAPDA KTVLPIPELD DAVVESFFSS STDSTPMFEY ENLEDNSKEW
         TSLFDNDIPV TTDDVSLADK AIESTEEVSL VPSNLEVSTT SFLPTPVLED AKLTQTRKVK
         KPNSVVKKSH HVGKDDESRL DHLGVVAYNR KQRSIPLSPI VPESSDPAAL KRARNTEAAR
         RSRARKLQRM KQLEDKVEEL LSKNYHLENE VARLKKLVGE R
    //

## Output file format

### Output files for usage example

### Graphics File: pepnet.ps

## Data files

None.

## Notes

None.

## References

None.

## Warnings

None.

## Diagnostic Error Messages

None.

## Exit status

It exits with a status of 0.

## Known bugs

None.

## See also

Program name | Description
--- | ---
abiview | Reads ABI file and display the trace
cirdna | Draws circular maps of DNA constructs
garnier | Predicts protein secondary structure
helixturnhelix | Report nucleic acid binding motifs
hmoment | Hydrophobic moment calculation
lindna | Draws linear maps of DNA constructs
pepcoil | Predicts coiled coil regions
pepwheel | Shows protein sequences as helices
prettyplot | Displays aligned sequences, with colouring and boxing
prettyseq | Output sequence with translated ranges
remap | Display a sequence with restriction cut sites, translation etc
seealso | Finds programs sharing group names
showalign | Displays a multiple sequence alignment
showdb | Displays information on the currently available databases
showfeat | Show features of a sequence
showseq | Display a sequence with features, translation etc
sixpack | Display a DNA sequence with 6-frame translation and ORFs
textsearch | Search sequence documentation text. SRS and Entrez are faster!
tmap | Displays membrane spanning regions

## Author(s)

Alan Bleasby (ableasby © hgmp.mrc.ac.uk)
HGMP-RC, Genome Campus, Hinxton, Cambridge CB10 1SB, UK

## History

Written (2000) - Alan Bleasby

## Target users

This program is intended to be used by everyone and everything, from naive users to embedded scripts.

## Comments