| ## EMBOSS: eprotdist

# Program eprotdist

## Function

Conversion of PHYLIP's protdist

## Description

EPROTDIST --Embossified program to compute distance matrix from protein sequences

Phylip protdist documentation.

## Usage

Here is a sample session with eprotdist using the data:-

       5   13
    Alpha     AACGTGGCCACAT
    Beta      AAGGTCGCCACAC
    Gamma     CAGTTCGCCACAA
    Delta     GAGATTTCCGCCT
    Epsilon   GAGATCTCCGCCC

    % eprotdist test

    Protein distance algorithm
    Output file [eprotdist.outfile]: stdout
    Method
           Pam : Dayhoff PAM matrix
           Kim : Kimura formula
           Cat : Categories model
    Choose the method to use [Pam]:
        5
    Alpha          0.00000  0.47285  0.88304  1.29841  2.12269
    Beta           0.47285  0.00000  0.45192  1.34185  0.84009
    Gamma          0.88304  0.45192  0.00000  1.30693  1.21582
    Delta          1.29841  1.34185  1.30693  0.00000  0.27536
    Epsilon        2.12269  0.84009  1.21582  0.27536  0.00000

## Command line arguments

       Mandatory qualifiers:
      [-msf]               seqset     File containing sequences
      [-outfile]           outfile    Output file name
       -method             list       Choose the method to use

       Optional qualifiers (* if not always prompted):
    *  -categ              list       Choose the categorie to use
    *  -gencode            list       Which genetic code
    *  -prob               float      Prob change category (1.0=easy)
    *  -tranrate           float      Transition/transversion ratio
    *  -[no]basefrequency  bool       Use empirical base frequencies
    *  -freqa              float      Frequency for A
    *  -freqc              float      Frequency for C
    *  -freqg              float      Frequency for G
    *  -freqt              float      Frequency for T/U
       -printdata          bool       Print out the data at start of run
       -progress           bool       Print indications of progress of run

       Advanced qualifiers: (none)

Mandatory qualifiers | Allowed values | Default
--- | --- | ---
[-msf] (Parameter 1) | File containing sequences | Readable sequences | Required
[-outfile] (Parameter 2) | Output file name | Output file | eprotdist.outfile
-method | Choose the method to use |
Pam | (Dayhoff PAM matrix)
Kim | (Kimura formula)
Cat | (Categories model)
| Pam
Optional qualifiers | Allowed values | Default
-categ | Choose the categorie to use |
G | (George/Hunt/Barker (Cys), (Met Val Leu Ileu), (Gly Ala Ser Thr Pro))
C | (Chemical (Cys Met), (Val Leu Ileu Gly Ala Ser Thr), (Pro))
H | (Hall (Cys), (Met Val Leu Ileu), (Gly Ala Ser Thr), (Pro))
| G
-gencode | Which genetic code |
U | (Universal)
M | (Mitochondrial)
V | (Vertebrate mitochondrial)
F | (Fly mitochondrial)
Y | (Yeast mitochondrial)
| U
-prob | Prob change category (1.0=easy) | Number from 0.000 to 1.000 | 0.457
-tranrate | Transition/transversion ratio | Number 0.000 or more | 2.0
-[no]basefrequency | Use empirical base frequencies | Yes/No | Yes
-freqa | Frequency for A | Number from 0.000 to 1.000 | 0.25
-freqc | Frequency for C | Number from 0.000 to 1.000 | 0.25
-freqg | Frequency for G | Number from 0.000 to 1.000 | 0.25
-freqt | Frequency for T/U | Number from 0.000 to 1.000 | 0.25
-printdata | Print out the data at start of run | Yes/No | No
-progress | Print indications of progress of run | Yes/No | No
Advanced qualifiers | Allowed values | Default
(none)

## Data files

## Notes

Multiple sequences sets not implemented yet!.

## References

## Warnings

## Diagnostics

## Exit status

## Known bugs

## See also

## Author(s)

(c) Copyright 1993 by Joseph Felsenstein. Permission is granted to copy this document provided that no fee is charged for it and that this copyright notice is not removed.

This application was modified for inclusion in EMBOSS by Ian Longden (il@sanger.ac.uk) Informatics Division, The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK.

## Priority

## Target users

## Comments